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KMID : 1134820180470010015
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Journal of the Korean Society of Food Science and Nutrition
2018 Volume.47 No. 1 p.15 ~ p.23
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Cytoprotective Effect of Water Extract of Vitis amurensis Root against Oxidative Stress in HaCaT Human Keratinocytes
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Choi Eun-Ok
Kwon Da-Hye
Hwang Young-Hoon
Jeong Jin-Woo
Nam Won-Sok
Lee Youn-Hee
Yang Hye-Kyung
Hwang Hye-Jin
Kim Byung-Woo
Lee Seung-Hun
Choi Yung-Hyun
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Abstract
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Root of Vitis amurensis Rupr., a wild-growing grape species, has been used as a traditional medicine for the treatment of various diseases in Asian countries, including Korea. Although several studies on the antioxidant effects of V. amurensis root extracts have been carried out, the underlying molecular mechanisms remain largely unresolved. In the present study, we investigated the cytoprotective efficacies of water extract of V. amurensis root (WEVA) against oxidative stress (hydrogen peroxide, H2O2) in HaCaT human keratinocytes. Our data show that treatment with WEVA prior to H2O2 exposure significantly increased HaCaT cell viability, indicating that exposure of HaCaT cells to WEVA conferred a protective effect against oxidative stress. WEVA preconditioning also effectively inhibited H2O2-induced apoptotic cell death by blocking degradation of poly (ADP-ribose) polymerase and down-regulation of pro-caspases-9 and -3. The anti-apoptotic effects of WEVA were associated with attenuation of mitochondrial dysfunction and normalization of expression changes of pro-apoptotic Bax and anti-apoptotic Bcl-2 in H2O2-stimulated HaCaT cells. In addition, WEVA abrogated H2O2-induced intracellular reactive oxygen species (ROS) accumulation and phosphorylation of histone H2AX at serine 139, a widely used marker of DNA damage. Moreover, WEVA increased the levels of hemeoxygenase-1 and NAD(P)H-quinone oxidoreductase-1, potent antioxidant enzymes associated with induction of nuclear transcription factor erythroid-2-like factor 2 (Nrf2). Thus, the present study demonstrates that WEVA protects human keratinocytes against oxidative stress-induced apoptosis and DNA damage, possibly through prevention of ROS generation and activation of Nrf2 signaling. Our data indicate that WEVA may potentially serve as an agent for treatment and prevention of skin disorders caused by oxidative stress.
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KEYWORD
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Vitis amurensis, antioxidant, apoptosis, ROS, DNA damage
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